Multicomponent blood analysis by near-infrared Raman spectroscopy.

نویسندگان

  • A J Berger
  • T W Koo
  • I Itzkan
  • G Horowitz
  • M S Feld
چکیده

We demonstrate the use of Raman spectroscopy to measure the concentration of many important constituents (analytes) in serum and whole blood samples at physiological concentration in vitro across a multipatient data set. A near-infrared (830-nm) diode laser generates Raman spectra that contain superpositions of Raman signals from different analytes. Calibrations for glucose, cholesterol, urea, and other analytes are developed by use of partial least-squares cross validation. We predict six analytes in serum with significant accuracy in a 66-patient data set, using 60-s spectra. The calibrations are shown to be fairly robust against system drift over the span of seven weeks. In whole blood, a preliminary analysis yields accurate predictions of some of the same analytes and also hematocrit. The results hold promise for potential medical applications.

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عنوان ژورنال:
  • Applied optics

دوره 38 13  شماره 

صفحات  -

تاریخ انتشار 1999